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This question already has an answer here:

At which point of hook execution is the currently activated theme's functions.php file loaded?

marked as duplicate by cjbj, Fayaz, Max Yudin, Robert hue, Mark Kaplun Apr 12 '17 at 10:32

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It is included from /wp-settings.php right after the action setup_theme. Line number 423 as of WordPress 4.7

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